First Report of Stolbur Phytoplasma Infection in Commercial Freesia hybrida Cultivars

Occurrence of Stolbur Phytoplasma Disease in Spreading Type Petunia hybrida Cultivars in Korea

In January 2012, spreading type petunia cv. Wave Pink plants showing an abnormal growth habit of sprouting unusual multiple plantlets from the lateral buds were collected from a greenhouse in Gwacheon, Gyeonggi Province, Korea. The presence of phytoplasma was investigated using PCR with the primer pairs P1/P6, and R16F1/R1 for nested-PCR. In the nested PCR, 1,096 bp PCR products were obtained, ...

First report: Grafting of Three Iranian Commercial Pomegranate Cultivars on Drought Tolerant Rootstocks

The objective of this study was to evaluate the stenting (grafted cutting) propagation of commercial pomegranate cultivars as scions on drought tolerant genotypes as rootstocks. The effect of drought stress on nine pomegranate rootstocks was analyzed. Cultivars including ‘Daneshgah 13’, ‘Daneshgah 32’ and ‘Daneshgah 8’ that were used as rootstocks showed the maximum drought tolerance among the ...

Generation and analysis of draft sequences of 'stolbur' phytoplasma from multiple displacement amplification templates.

Phytoplasma-associated diseases are reported for more than 1,000 plant species worldwide. Only a few genome sequences are available in contrast to the economical importance of these bacterial pathogens. A new strategy was used to retrieve phytoplasma strain-specific genome data. Multiple displacement amplification was performed on DNA obtained from <3 g of plant tissue from tobacco and parsley ...

Stolbur phytoplasma genome survey achieved using a suppression subtractive hybridization approach with high specificity.

Phytoplasmas are unculturable bacterial plant pathogens transmitted by phloem-feeding hemipteran insects. DNA of phytoplasmas is difficult to purify because of their exclusive phloem location and low abundance in plants. To overcome this constraint, suppression subtractive hybridization (SSH) was modified and used to selectively amplify DNA of the stolbur phytoplasma infecting a periwinkle plan...

First report of infection with Clostridium oedematiens type D in sheep in Iran